TNF Alpha Antibody (clone MM0N1, Preservative Free)

LifeSpan BioSciences

TNF Alpha Antibody (clone MM0N1, Preservative Free)

14

Block: In a functional ELISA which immobilized recombinant Human TNFR1 at 1ug/ml (100 microL/well) in the plate, the Mouse Anti-Human TNFa Monoclonal Antibody can block the binding of 0.2 ug/ml of biotinylated Human TNFa to human TNFR1, the EC50 is 0.74 ug/ml;In a functional ELISA which immobilized recombinant Human TNFR2 at 2ug/ml (100 microL/well) in the plate, the Mouse Anti-Human TNFa Monoclonal Antibody can block the binding of 0.2 ug/ml of biotinylated Human TNFa to human TNFR1, the EC50 is 0.82 ug/ml. Neutralization: The neutralization activity of antibody is measured by its ability to neutralize TNFa induced cytotoxicity in the L-929 mouse fibroblast cell line. The Neutralization titer (IC50) is typically 13. 5-54 ng/mL in the presence of 2 ng/mL Recombinant Human TNFa and 1 ug/ml actinomycin D. Please Note: Optimal concentrations/dilutions should be determined by the end user.. Block (0.2 µg/ml), ELISA, Neut Block: In a functional ELISA which immobilized recombinant Human TNFR1 at 1ug/ml (100 µL/well) in the plate, the Mouse Anti-Human TNFa Monoclonal Antibody can block the binding of 0.2 ug/ml of biotinylated Human TNFa to human TNFR1, the EC50 is 0.74 ug/ml;In a functional ELISA which immobilized recombinant Human TNFR2 at 2ug/ml (100 µL/well) in the plate, the Mouse Anti-Human TNFa Monoclonal Antibody can block the binding of 0.2 ug/ml of biotinylated Human TNFa to human TNFR1, the EC50 is 0.82 ug/ml. Neutralization: The neutralization activity of antibody is measured by its ability to neutralize TNFa induced cytotoxicity in the L-929 mouse fibroblast cell line. The Neutralization titer (IC50) is typically 13. 5-54 ng/mL in the presence of 2 ng/mL Recombinant Human TNFa and 1 ug/ml actinomycin D. Please Note: Optimal concentrations/dilutions should be determined by the end user.

ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

MM0N1

Unconjugated

...

TNF

tumor necrosis factor

DIF, IMD127, TNF-alpha, TNFA, TNFSF2, TNLG1F, tumor necrosis factor, APC1 protein, TNF, macrophage-derived, TNF, monocyte-derived, TNF-a, tumor necrosis factor ligand 1F, tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha, tumor necrotic factor alpha

Mouse

IgG1

-20°C or -80°C,2°C to 8°C

12352203