Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody [HL1579] (GTX637058) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody [HL1579] (GTX637058) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637058/GTX637058_44767_20220819_WB_treatment_TPA_LPS_BFA_22082402_989.webp "Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody [HL1579] (GTX637058) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Indirect ELISA analysis was performed by coating the plate with recombinant HEK293 expressed, mouse TNF-alpha Protein (209.42-3.27 nM). Coated protein was probed with TNF alpha antibody [HL1579] (GTX637058) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637058/GTX637058_44767_20241011_ELISA_Indirect_24101600_377.webp "Indirect ELISA analysis was performed by coating the plate with recombinant HEK293 expressed, mouse TNF-alpha Protein (209.42-3.27 nM). Coated protein was probed with TNF alpha antibody [HL1579] (GTX637058) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.")
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TNF alpha antibody [HL1579] (GTX637058) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
TNF alpha antibody [HL1579]
GTX637058
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetTNF
Overview
- SupplierGeneTex
- Product NameTNF alpha antibody [HL1579]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1579
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID7124
- Target nameTNF
- Target descriptiontumor necrosis factor
- Target synonymsDIF, IMD127, TNF-alpha, TNFA, TNFSF2, TNLG1F, tumor necrosis factor, APC1 protein, TNF, macrophage-derived, TNF, monocyte-derived, TNF-a, tumor necrosis factor ligand 1F, tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha, tumor necrotic factor alpha
- HostRabbit
- IsotypeIgG
- Protein IDP01375
- Protein NameTumor necrosis factor
- Scientific DescriptionThis gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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![Sandwich ELISA detection of recombinant 293T expressed, human Fc-tagged TNF alpha (54-233 a.a.) using antibodies as below. Capture: TNF alpha antibody [HL2363] (GTX638569) (5 μg/mL) Detection: HRP-conjugated TNF alpha antibody [HL2361] (GTX638567) (1 μg/mL) Please notice that GTX638567 needs to be conjugated to HRP to function as the detection antibody when paired with GTX638569. Please contact us for custom HRP-conjugated antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638567/GTX638567_T-45033_20230519_ELISA_PAIR_2_23052301_628.webp)
Product group Antibodies
TNF alpha antibody [HL2361]GTX638567
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- SizePrice