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Mouse lymph node probed with TNF alpha Polyclonal Antibody (bs-10802R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Mouse lymph node probed with TNF alpha Polyclonal Antibody (bs-10802R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Mouse lymph node probed with TNF alpha Polyclonal Antibody (bs-10802R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

TNF alpha Polyclonal Antibody

BS-10802R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTNF
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Overview

  • Supplier
    Bioss
  • Product Name
    TNF alpha Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID7124
  • Target name
    TNF
  • Target description
    tumor necrosis factor
  • Target synonyms
    DIF, IMD127, TNF-alpha, TNFA, TNFSF2, TNLG1F, tumor necrosis factor, APC1 protein, TNF, macrophage-derived, TNF, monocyte-derived, TNF-a, tumor necrosis factor ligand 1F, tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha, tumor necrotic factor alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01375
  • Protein Name
    Tumor necrosis factor
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Meng HW, You HM, Yang Y, et al. 4-Methylcoumarin-[5,6-g]-hesperetin attenuates inflammatory responses in alcoholic hepatitis through PPAR-γ activation. Toxicology. 2019,421:9-21. doi: 10.1016/j.tox.2019.04.004
    Read this paper
  • Cheng XY, Liu JD, Lu XY, et al. miR-203 Inhibits Alcohol-Induced Hepatic Steatosis by Targeting Lipin1. Front Pharmacol. 2018,9:275. doi: 10.3389/fphar.2018.00275
    Read this paper

Datasheet

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Figure 1. Western blot analysis of TNF alpha using anti-TNF alpha antibody (A00002-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human TNF alpha protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # A00002-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 17KD. The expected band size for TNF alpha is at 17KD.
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