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L1 mouse intestine lysates probed with Anti TNFR1/TNF Receptor I Polyclonal Antibody, Unconjugated (bs-2941R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 40kD/26kD. Observed band size:40kD/26kD.\n
L1 mouse intestine lysates probed with Anti TNFR1/TNF Receptor I Polyclonal Antibody, Unconjugated (bs-2941R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 40kD/26kD. Observed band size:40kD/26kD.\n
L1 mouse intestine lysates probed with Anti TNFR1/TNF Receptor I Polyclonal Antibody, Unconjugated (bs-2941R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 40kD/26kD. Observed band size:40kD/26kD.\n

TNFR1 Polyclonal Antibody

BS-2941R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetTNFRSF1A
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    TNF Receptor I Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID7132
  • Target name
    TNFRSF1A
  • Target description
    TNF receptor superfamily member 1A
  • Target synonyms
    CD120a; FPF; p55; p55-R; p60; TBP1; TNFAR; TNF-R; TNFR1; TNF-R1; TNFR55; TNF-R55; TNFR60; TNF-RI; TNF-R-I; TNFR-I; tumor necrosis factor binding protein 1; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor type 1; tumor necrosis factor-alpha receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP19438
  • Protein Name
    Tumor necrosis factor receptor superfamily member 1A
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Tumor necrosis factor alpha inhibitors have no effect on a human T-lymphotropic virus type-I (HTLV-I)-infected cell line from patients with HTLV-I-associated myelopathy. Fukui S et al., 2017 Feb 3, BMC Immunol
    Read more
  • Macrolide antibiotics differentially influence human HepG2 cytotoxicity and modulate intrinsic/extrinsic apoptotic pathways in rat hepatocellular carcinoma model. Abdel-Hamid NI et al., 2017 Apr, Naunyn Schmiedebergs Arch Pharmacol
    Read more
  • Inhibitory effect of Zanthoxylum bungeanum seed oil on ovalbumin-induced lung inflammation in a murine model of asthma. Wang JQ et al., 2016 May, Mol Med Rep
    Read more

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Figure 1. Western blot analysis of TNF Receptor I using anti-TNF Receptor I antibody (A00294-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate, Lane 2: human K562 whole cell lysate, Lane 3: human Caco-2 whole cell lysate, Lane 4: rat liver tissue lysates, Lane 5: mouse small intestine tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF Receptor I antigen affinity purified polyclonal antibody (Catalog # A00294-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF Receptor I at approximately 50KD. The expected band size for TNF Receptor I is at 50KD.
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