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ProductsBack/TNFRSF1A Antibody
Western Blot Positive WB detected in: A549 whole cell lysate, PC-3 whole cell lysate All lanes: TNFRSF1A antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 51, 39, 26, 25 kDa Observed band size: 51 kDa
Western Blot Positive WB detected in: A549 whole cell lysate, PC-3 whole cell lysate All lanes: TNFRSF1A antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 51, 39, 26, 25 kDa Observed band size: 51 kDa
Western Blot Positive WB detected in: A549 whole cell lysate, PC-3 whole cell lysate All lanes: TNFRSF1A antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 51, 39, 26, 25 kDa Observed band size: 51 kDa

TNFRSF1A Antibody

CSB-PA023977LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTNFRSF1A
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Overview

  • Supplier
    Cusabio
  • Product Name
    TNFRSF1A Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7132
  • Target name
    TNFRSF1A
  • Target description
    TNF receptor superfamily member 1A
  • Target synonyms
    CD120a; FPF; p55; p55-R; p60; TBP1; TNFAR; TNF-R; TNFR1; TNF-R1; TNFR55; TNF-R55; TNFR60; TNF-RI; TNF-R-I; TNFR-I; tumor necrosis factor binding protein 1; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor type 1; tumor necrosis factor-alpha receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP19438
  • Protein Name
    Tumor necrosis factor receptor superfamily member 1A
  • Scientific Description
    Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TNF Receptor I using anti-TNF Receptor I antibody (A00294-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate, Lane 2: human K562 whole cell lysate, Lane 3: human Caco-2 whole cell lysate, Lane 4: rat liver tissue lysates, Lane 5: mouse small intestine tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF Receptor I antigen affinity purified polyclonal antibody (Catalog # A00294-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF Receptor I at approximately 50KD. The expected band size for TNF Receptor I is at 50KD.
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