
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Transgelin antibody (GTX113561) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Transgelin antibody
GTX113561
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Human, Mouse, Rat
TargetTAGLN
Overview
- SupplierGeneTex
- Product NameTransgelin antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.33 mg/ml
- ConjugateUnconjugated
- Gene ID6876
- Target nameTAGLN
- Target descriptiontransgelin
- Target synonymsSM22, SM22-alpha, SMCC, TAGLN1, TGLN, WS3-10, transgelin, 22 kDa actin-binding protein, epididymis secretory sperm binding protein, smooth muscle protein 22-alpha
- HostRabbit
- IsotypeIgG
- Protein IDQ01995
- Protein NameTransgelin
- Scientific DescriptionThe protein encoded by this gene is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
- ReactivityBovine, Human, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161



![Transgelin antibody detects Transgelin protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Transgelin protein stained by Transgelin antibody (GTX113561) diluted at 1:100. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. Blue: Hoechst 33342 staining. Transgelin antibody detects Transgelin protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Transgelin protein stained by Transgelin antibody (GTX113561) diluted at 1:100. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX113561/GTX113561_40163_20150410_IFA_w_23060501_680.webp)













![Transgelin antibody detects Transgelin protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Transgelin protein stained by Transgelin antibody (GTX101608) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101608/GTX101608_40058_20150410_IFA_w_23060100_843.webp)