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U2AF2 Recombinant Antibody

BSM-62036R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetU2AF2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    U2AF2 Recombinant Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF(ICC)(1:50-200), IF()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID11338
  • Target name
    U2AF2
  • Target description
    U2 small nuclear RNA auxiliary factor 2
  • Target synonyms
    hU2AF65; splicing factor U2AF 65 kDa subunit; U2 (RNU2) small nuclear RNA auxiliary factor 2; U2 auxiliary factor 65 kDa subunit; U2 small nuclear ribonucleoprotein auxiliary factor (65kD); U2 snRNP auxiliary factor large subunit; U2AF65
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP26368
  • Protein Name
    Splicing factor U2AF 65 kDa subunit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of U2AF2 using anti-U2AF2 antibody (A03639-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat spleen tissue lysates, Lane 6: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-U2AF2 antigen affinity purified polyclonal antibody (Catalog # A03639-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for U2AF2 at approximately 65KD. The expected band size for U2AF2 is at 65KD.
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