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U2AF2 / U2AF65 Antibody

LS-C331774
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetU2AF2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    U2AF2 / U2AF65 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 53kDa, while the observed MW by Western blot was 115kDa.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IF (1:50 - 1:100), IHC (1:50 - 1:200), IP (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 53kDa, while the observed MW by Western blot was 115kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2.82 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID11338
  • Target name
    U2AF2
  • Target description
    U2 small nuclear RNA auxiliary factor 2
  • Target synonyms
    DEVDFB, U2AF65, splicing factor U2AF 65 kDa subunit, U2 (RNU2) small nuclear RNA auxiliary factor 2, U2 auxiliary factor 65 kDa subunit, U2 small nuclear ribonucleoprotein auxiliary factor (65kD), U2 snRNP auxiliary factor large subunit, hU2AF65
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of U2AF2 using anti-U2AF2 antibody (A03639-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat spleen tissue lysates, Lane 6: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-U2AF2 antigen affinity purified polyclonal antibody (Catalog # A03639-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for U2AF2 at approximately 65KD. The expected band size for U2AF2 is at 65KD.
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