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Western blot using GeneTex's affinity purified anti-UBC13 antibody shows detection of UBC13 protein in human small intestine lysate (lane 1) but not in mouse thymus lysate (lane 2). The heavily stained band in lane 1 (arrowhead) indicates this particular gel was overloaded with protein. The identity of minor reactive bands is unknown but could represent E2 complexes. Each lane contains approximately 20 μg of lysate. Primary antibody was used at a 1:500 dilution. The membrane was washed and reacted with a 1:10,000 dilution of Alexa FluorTM 680 conjugated rabbit anti-Goat IgG. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (lane M). Other detection systems will yield similar results.
Western blot using GeneTex's affinity purified anti-UBC13 antibody shows detection of UBC13 protein in human small intestine lysate (lane 1) but not in mouse thymus lysate (lane 2). The heavily stained band in lane 1 (arrowhead) indicates this particular gel was overloaded with protein. The identity of minor reactive bands is unknown but could represent E2 complexes. Each lane contains approximately 20 μg of lysate. Primary antibody was used at a 1:500 dilution. The membrane was washed and reacted with a 1:10,000 dilution of Alexa FluorTM 680 conjugated rabbit anti-Goat IgG. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (lane M). Other detection systems will yield similar results.
Western blot using GeneTex's affinity purified anti-UBC13 antibody shows detection of UBC13 protein in human small intestine lysate (lane 1) but not in mouse thymus lysate (lane 2). The heavily stained band in lane 1 (arrowhead) indicates this particular gel was overloaded with protein. The identity of minor reactive bands is unknown but could represent E2 complexes. Each lane contains approximately 20 μg of lysate. Primary antibody was used at a 1:500 dilution. The membrane was washed and reacted with a 1:10,000 dilution of Alexa FluorTM 680 conjugated rabbit anti-Goat IgG. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (lane M). Other detection systems will yield similar results.

UBE2N antibody

GTX48485
GeneTex
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetUBE2N
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Overview

  • Supplier
    GeneTex
  • Product Name
    UBE2N antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:2000. ELISA: 1:5000-1:25000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.25 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7334
  • Target name
    UBE2N
  • Target description
    ubiquitin conjugating enzyme E2 N
  • Target synonyms
    HEL-S-71, UBC13, UBCHBEN, UBCHBEN; UBC13, UbcH-ben, UbcH13, ubiquitin-conjugating enzyme E2 N, E2 ubiquitin-conjugating enzyme N, bendless-like ubiquitin conjugating enzyme, epididymis secretory protein Li 71, ubiquitin carrier protein N, ubiquitin conjugating enzyme E2N, ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast), ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13), ubiquitin-protein ligase N, yeast UBC13 homolog
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDP61088
  • Protein Name
    Ubiquitin-conjugating enzyme E2 N
  • Scientific Description
    UBC13 is a member of the E2 ubiquitin-conjugating enzyme family also known as Ubiquitin-conjugating enzyme E2 N, Ubiquitin-protein ligase N, Ubiquitin carrier protein N and Bendless-like ubiquitin-conjugating enzyme. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Ubc13/UBE2N using anti-Ubc13/UBE2N antibody (A02462-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: rat C6 whole cell lysates, Lane 8: mouse thymus tissue lysates, Lane 9: mouse small intestine tissue lysates, Lane 10: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ubc13/UBE2N antigen affinity purified polyclonal antibody (Catalog # A02462-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ubc13/UBE2N at approximately 17 kDa. The expected band size for Ubc13/UBE2N is at 17 kDa.
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