Bio-Connect

Ubiquitin Activating Enzyme E1 [UBA1; UBE1] (human) (rec.) (untagged)

Research Use Only
SBB-CE0011
South Bay Bio
Protein IDP22314
Product group Proteins / Signaling Molecules
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    South Bay Bio
  • Product Name
    Ubiquitin Activating Enzyme E1 [UBA1; UBE1] (human) (rec.) (untagged)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Estimated Purity
    >95%
  • Formulation
    Liquid
  • Protein IDP22314
  • Protein Name
    Ubiquitin-like modifier-activating enzyme 1
  • Scientific Description
    Protein. Human UBA1 (aa1-2-1058)is untagged. Source: Sf21 cells. Formulation: Liquid. In 50mM HEPES pH 7.5, 150mM sodium chloride, 10% glycerol, 2mM TCEP. Purity: >95% (SDS-PAGE). UBA1, the canonical ubiquitin E1 activating enzyme is a 118kDa protein, which forms a homodimer in its active state and catalyzes the first step of the ubiquitin conjugation cascade. It activates ubiquitin in an ATP-dependent mechanism where ATP is hydrolyzed to AMP and PPi, a ubiquitin C-terminal adenylate intermediate is formed, then transferred to the E1s active site cysteine through a thioester bond. This thioester is then transferable to an E2 conjugating enzymes active site cysteine. - UBA1, the canonical ubiquitin E1 activating enzyme is a 118kDa protein, which forms a homodimer in its active state and catalyzes the first step of the ubiquitin conjugation cascade. It activates ubiquitin in an ATP-dependent mechanism where ATP is hydrolyzed to AMP and PPi, a ubiquitin C-terminal adenylate intermediate is formed, then transferred to the E1s active site cysteine through a thioester bond. This thioester is then transferable to an E2 conjugating enzymes active site cysteine.
  • Storage Instruction
    -80°C
  • UNSPSC
    12352202

Related products

Product group Proteins / Signaling Molecules
South Bay Bio
Protein IDP22314
  • SizePrice
Thioester Activity Assay: UBE2D3 (UbcH5c) forms a thioester with Ub in an ATP-dependent manner and the bond can be reduced with addition of access DTT. Confirms the activity of UBE2D3.
Product group Proteins / Signaling Molecules
South Bay Bio
Protein IDP61077
  • SizePrice
Thioester Activity Assay: UBE2L3 (UbcH7) forms a thioester with Ub in an ATP-dependent manner and the bond can be reduced with addition of access DTT. Confirms the activity of UBE2L3.
Product group Proteins / Signaling Molecules
South Bay Bio
Protein IDP68036
  • SizePrice
Thioester Activity Assay: UBE2D1 (UbcH5a) forms a thioester with Ub in an ATP-dependent manner and the bond can be reduced with addition of access DTT. Confirms the activity of UBE2D1.
Product group Proteins / Signaling Molecules
South Bay Bio
Protein IDP51668
  • SizePrice
Thioester Activity Assay: UBE2K forms a thioester with Ub in an ATP-dependent manner and the bond can be reduced with addition of excess DTT. The thioester assay also shows di-ubiquitin formation with addition of ATP. This confirms the activity of
Product group Proteins / Signaling Molecules
South Bay Bio
Protein IDP61086
  • SizePrice
Thioester Activity Assay: UBE2D2 (UbcH5b) forms a thioester with UB in an ATP dependent manner and the bond can be reduced with addition of access DTT. Confirms the activity of UBE2D2.
Product group Proteins / Signaling Molecules
South Bay Bio
Protein IDP62837
  • SizePrice
Parkin (E3 Ligase) (human) (rec.) (His)
Product group Proteins / Signaling Molecules
South Bay Bio
  • SizePrice
Ubiquitin Activating Enzyme E1 [UBA1; UBE1] (human) (rec.) (His)
Product group Proteins / Signaling Molecules
South Bay Bio
Protein IDP22314
  • SizePrice
Michaelis-Menten Kinetics: Ubiquitin Rhodamine 110 serially diluted from 1.6 to 0.05microM was digested with 30pM UcHL3 over time. The assay was carried out in a reaction buffer of 50mM HEPES pH 7.5, 100mM NaCl, 1mM TCEP, 0.1mg/ml BSA, at 25°C. Initial
Product group Proteins / Signaling Molecules
South Bay Bio
Protein IDP15374
  • SizePrice
Product group Proteins / Signaling Molecules
South Bay Bio
Protein IDP0C6U8
  • SizePrice