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ProductsBack/VCP antibody [N1N2], N-term

VCP antibody [N1N2], N-term

GTX113030
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetVCP
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Overview

  • Supplier
    GeneTex
  • Product Name
    VCP antibody [N1N2], N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:10000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.3 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7415
  • Target name
    VCP
  • Target description
    valosin containing protein
  • Target synonyms
    CDC48, FTDALS6, TERA, p97, transitional endoplasmic reticulum ATPase, 15S Mg(2+)-ATPase p97 subunit, TER ATPase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55072
  • Protein Name
    Transitional endoplasmic reticulum ATPase
  • Scientific Description
    The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Yamamoto M, Fujiwara N. Protein phosphatase 6 regulates trametinib sensitivity, a mitogen-activated protein kinase kinase (MEK) inhibitor, by regulating MEK1/2-ERK1/2 signaling in canine melanoma cells. J Vet Med Sci. 2023,85(9):977-984. doi: 10.1292/jvms.23-0274
    Read this paper
  • Hancock JL, Kalimutho M, Straube J, et al. COMMD3 loss drives invasive breast cancer growth by modulating copper homeostasis. J Exp Clin Cancer Res. 2023,42(1):90. doi: 10.1186/s13046-023-02663-8
    Read this paper
  • Guettsches AK, Meyer N, Zahedi RP, et al. FYCO1 Increase and Effect of Arimoclomol-Treatment in Human VCP-Pathology. Biomedicines. 2022,10(10). doi: 10.3390/biomedicines10102443
    Read this paper
  • Tsunedomi R, Yoshimura K, Kimura Y, et al. Elevated expression of RAB3B plays important roles in chemoresistance and metastatic potential of hepatoma cells. BMC Cancer. 2022,22(1):260. doi: 10.1186/s12885-022-09370-1
    Read this paper
  • Kitamura N, Fujiwara N, Hayakawa K, et al. Protein phosphatase 6 promotes neurite outgrowth by promoting mTORC2 activity in N2a cells. J Biochem. 2021,170(1):131-138. doi: 10.1093/jb/mvab028
    Read this paper
  • Fujiwara Y, Tsunedomi R, Yoshimura K, et al. Pancreatic Cancer Stem-Like Cells With High Calreticulin Expression Associated With Immune Surveillance. Pancreas. 2021,50(3):405-413. doi: 10.1097/MPA.0000000000001772
    Read this paper
  • Fujiwara N, Shibutani S, Ohama T, et al. Protein phosphatase 6 dissociates the Beclin 1/Vps34 complex and inhibits autophagy. Biochem Biophys Res Commun. 2021,552:191-195. doi: 10.1016/j.bbrc.2021.02.136
    Read this paper
  • Fujimoto T, Tsunedomi R, Matsukuma S, et al. Cathepsin B is highly expressed in pancreatic cancer stem-like cells and is associated with patients' surgical outcomes. Oncol Lett. 2021,21(1):30. doi: 10.3892/ol.2020.12291
    Read this paper
  • Fujiwara N, Shibutani S, Sakai Y, et al. Autophagy regulates levels of tumor suppressor enzyme protein phosphatase 6. Cancer Sci. 2020,111(12):4371-4380. doi: 10.1111/cas.14662
    Read this paper
  • Ikeda S, Tsuji S, Ohama T, et al. Involvement of PP2A methylation in the adipogenic differentiation of bone marrow-derived mesenchymal stem cell. J Biochem. 2020,168(6):643-650. doi: 10.1093/jb/mvaa077
    Read this paper

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Figure 1. Western blot analysis of VCP using anti-VCP antibody (A00610-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: Monkey COS-7 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human placenta tissue lysates, Lane 8: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCP antigen affinity purified polyclonal antibody (Catalog # A00610-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VCP at approximately 97 kDa. The expected band size for VCP is at 89 kDa.
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