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ProductsBack/VCP antibody [N3C2], Internal
Sample (30 μg of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7.5% SDS PAGE GTX113099 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sample (30 μg of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7.5% SDS PAGE GTX113099 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sample (30 μg of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7.5% SDS PAGE GTX113099 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

VCP antibody [N3C2], Internal

GTX113099
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetVCP
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Overview

  • Supplier
    GeneTex
  • Product Name
    VCP antibody [N3C2], Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:20000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7415
  • Target name
    VCP
  • Target description
    valosin containing protein
  • Target synonyms
    CDC48, FTDALS6, TERA, p97, transitional endoplasmic reticulum ATPase, 15S Mg(2+)-ATPase p97 subunit, TER ATPase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55072
  • Protein Name
    Transitional endoplasmic reticulum ATPase
  • Scientific Description
    The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of VCP using anti-VCP antibody (A00610-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: Monkey COS-7 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human placenta tissue lysates, Lane 8: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCP antigen affinity purified polyclonal antibody (Catalog # A00610-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VCP at approximately 97 kDa. The expected band size for VCP is at 89 kDa.
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