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ProductsBack/VIL1 / Villin Antibody (aa770-799)

VIL1 / Villin Antibody (aa770-799)

LS-C407669
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetVIL1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    VIL1 / Villin Antibody (aa770-799)
  • Delivery Days Customer
    23
  • Application Supplier Note
    WB: The detection limit for Villin is approximately 0.1 ng/lane under reducing conditions. IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.. IHC, IHC-P (0.5 - 1 µg/ml), WB (0.1 - 0.5 µg/ml) WB: The detection limit for Villin is approximately 0.1 ng/lane under reducing conditions. IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7429
  • Target name
    VIL1
  • Target description
    villin 1
  • Target synonyms
    D2S1471, VIL, villin-1
  • Host
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Villin using anti-Villin antibody (PB9457). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Intestine Tissue Lysate at 50ug Lane 2: Mouse Kidney Tissue Lysate at 50ug Lane 3: RH35 Whole Cell Lysate at 40ug Lane 4: HEPG2 Whole Cell Lysate at 40ug, Lane 5: MCF-7 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Villin antigen affinity purified polyclonal antibody (Catalog # PB9457) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Villin at approximately 93 kDa. The expected band size for Villin is at 93 kDa.
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