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Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Villin antibody [HL1756] (GTX637406) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Villin antibody [HL1756] (GTX637406) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Villin antibody [HL1756] (GTX637406) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Villin antibody [HL1756]

GTX637406
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetVIL1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Villin antibody [HL1756]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL1756
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7429
  • Target name
    VIL1
  • Target description
    villin 1
  • Target synonyms
    D2S1471, VIL, villin-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09327
  • Protein Name
    Villin-1
  • Scientific Description
    This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Villin using anti-Villin antibody (PB9457). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Intestine Tissue Lysate at 50ug Lane 2: Mouse Kidney Tissue Lysate at 50ug Lane 3: RH35 Whole Cell Lysate at 40ug Lane 4: HEPG2 Whole Cell Lysate at 40ug, Lane 5: MCF-7 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Villin antigen affinity purified polyclonal antibody (Catalog # PB9457) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Villin at approximately 93 kDa. The expected band size for Villin is at 93 kDa.
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