Detection of endogenous Vimentin: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab Vim-11H6 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: HeLa; lane 2: HepG2; lane 3: HEK293; lane 4: SH-SY5Y; lane 5: MDCK; lane 6: PC12; lane 7: CMT 93; lane 8: Neuro 2A; lane 9: 3T3
Vimentin (VIM) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11H6]
AM00158PU-N
Overview
- SupplierOriGene
- Product NameVimentin (VIM) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11H6]
- Delivery Days Customer14
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID11H6
- Gene ID7431
- Target nameVIM
- Target descriptionvimentin
- Target synonymsepididymis secretory sperm binding protein; vimentin
- HostMouse
- IsotypeIgG
- Scientific DescriptionVimentin (VIM) (incl. pos. control) mouse monoclonal antibody, clone 11H6, Purified
- ReactivityCanine, Human, Mouse
- UNSPSC12352203