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Vitamin D Binding Protein (DBP) Monoclonal Antibody

CAU33856
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityRat
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Overview

  • Supplier
    Biomatik
  • Product Name
    Vitamin D Binding Protein (DBP) Monoclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    C2
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP04276
  • Protein Name
    Vitamin D-binding protein
  • Scientific Description
    The Vitamin D Binding Protein (DBP) Monoclonal Antibody (Species: Rat) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity
    Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Vitamin D Binding protein using anti-Vitamin D Binding protein antibody (A03364-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human A431 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vitamin D Binding protein antigen affinity purified polyclonal antibody (Catalog # A03364-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vitamin D Binding protein at approximately 53KD. The expected band size for Vitamin D Binding protein is at 53KD.
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