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WB analysis of human placenta (A) and human adrenal gland (B) lysate using GTX89638 VMAT2 antibody, C-term. Dilution : 1microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human placenta (A) and human adrenal gland (B) lysate using GTX89638 VMAT2 antibody, C-term. Dilution : 1microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human placenta (A) and human adrenal gland (B) lysate using GTX89638 VMAT2 antibody, C-term. Dilution : 1microg/ml Loading : 35microg protein in RIPA buffer

VMAT2 antibody, C-term

GTX89638
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetSLC18A2
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Overview

  • Supplier
    GeneTex
  • Product Name
    VMAT2 antibody, C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1-3microg/ml. IHC-P: 5microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6571
  • Target name
    SLC18A2
  • Target description
    solute carrier family 18 member A2
  • Target synonyms
    monoamine neurotransmitter transporter; PKDYS2; solute carrier family 18 (vesicular monoamine transporter), member 2; SVAT; SVMT; synaptic vesicle amine transporter, brain; synaptic vesicle monoamine transporter, brain; synaptic vesicular amine transporter; VAT2; vesicle monoamine transporter type 2; vesicle monoamine/H+ antiporter; vesicular amine transporter 2; VMAT2
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDQ05940
  • Protein Name
    Synaptic vesicular amine transporter
  • Scientific Description
    This gene encodes an transmembrane protein that functions as an ATP-dependent transporter of monoamines, such as dopamine, norepinephrine, serotonin, and histamine. This protein transports amine neurotransmitters into synaptic vesicles. Polymorphisms in this gene may be associated with schizophrenia, bipolar disorder, and other neurological/psychiatric ailments. [provided by RefSeq, Jun 2018]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Female Sex and Brain-Selective Estrogen Benefit alpha-Synuclein Tetramerization and the PD-like Motor Syndrome in 3K Transgenic Mice. Rajsombath MM et al., 2019 Sep 18, J Neurosci
    Read more

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Figure 1. Western blot analysis of VMAT2/SLC18A2 using anti-VMAT2/SLC18A2 antibody (A02232-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VMAT2/SLC18A2 antigen affinity purified polyclonal antibody (Catalog # A02232-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VMAT2/SLC18A2 at approximately 75 kDa. The expected band size for VMAT2/SLC18A2 is at 56 kDa.
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