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ProductsBack/XPA antibody [5F12]
ICC/IF analysis of GM0637 cells irradiated with UV at 20 J/m2 using GTX00704 XPA antibody [5F12]. Dilution : 1:100
ICC/IF analysis of GM0637 cells irradiated with UV at 20 J/m2 using GTX00704 XPA antibody [5F12]. Dilution : 1:100
ICC/IF analysis of GM0637 cells irradiated with UV at 20 J/m2 using GTX00704 XPA antibody [5F12]. Dilution : 1:100

XPA antibody [5F12]

GTX00704
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, Neutralisation/Blocking
Product group Antibodies
TargetXPA
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Overview

  • Supplier
    GeneTex
  • Product Name
    XPA antibody [5F12]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5F12
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7507
  • Target name
    XPA
  • Target description
    XPA, DNA damage recognition and repair factor
  • Target synonyms
    XP1, XPAC, DNA repair protein complementing XP-A cells, xeroderma pigmentosum group A-complementing protein, xeroderma pigmentosum, complementation group A
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP23025
  • Protein Name
    DNA repair protein complementing XP-A cells
  • Scientific Description
    This gene encodes a zinc finger protein plays a central role in nucleotide excision repair (NER), a specialized type of DNA repair. NER is responsible for repair of UV radiation-induced photoproducts and DNA adducts induced by chemical carcinogens and chemotherapeutic drugs. The encoded protein interacts with DNA and several NER proteins, acting as a scaffold to assemble the NER incision complex at sites of DNA damage. Mutations in this gene cause Xeroderma pigmentosum complementation group A (XP-A), an autosomal recessive skin disorder featuring hypersensitivity to sunlight and increased risk for skin cancer. [provided by RefSeq, Aug 2017]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Tanaka K, Miura N, Satokata I, et al. Analysis of a human DNA excision repair gene involved in group A xeroderma pigmentosum and containing a zinc-finger domain. Nature. 1990,348(6296):73-6.
    Read this paper

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Figure 1. Western blot analysis of XPA using anti-XPA antibody (A01182-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XPA antigen affinity purified polyclonal antibody (Catalog # A01182-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XPA at approximately 39 kDa. The expected band size for XPA is at 31 kDa.
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