![WB analysis of HeLa (1), C6 (2) and Cos7 (3) cell lysate using GTX60554 YAP1 antibody [1A12]. WB analysis of HeLa (1), C6 (2) and Cos7 (3) cell lysate using GTX60554 YAP1 antibody [1A12].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60554/GTX60554_20170912_WB_w_23061123_663.webp)
WB analysis of HeLa (1), C6 (2) and Cos7 (3) cell lysate using GTX60554 YAP1 antibody [1A12].
YAP1 antibody [1A12]
GTX60554
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Monkey, Rat
TargetYAP1
Overview
- SupplierGeneTex
- Product NameYAP1 antibody [1A12]
- Delivery Days Customer9
- Application Supplier NoteWB: 1/500 - 1/2000. IHC-P: 1/200 - 1/1000. FACS: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID1A12
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID10413
- Target nameYAP1
- Target descriptionYes1 associated transcriptional regulator
- Target synonymsCOB1, YAP, YAP-1, YAP2, YAP65, YKI, transcriptional coactivator YAP1, 65 kDa Yes-associated protein, Yes associated protein 1, protein yorkie homolog, yes-associated protein 2, yes-associated protein YAP65 homolog, yorkie homolog
- HostMouse
- IsotypeIgG1
- Protein IDP46937
- Protein NameTranscriptional coactivator YAP1
- Scientific DescriptionThis gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Monkey, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Chang HA, Ou Yang RZ, Su JM, et al. YAP nuclear translocation induced by HIF-1α prevents DNA damage under hypoxic conditions. Cell Death Discov. 2023,9(1):385. doi: 10.1038/s41420-023-01687-5Read this paper
![FACS analysis of HeLa cells using GTX60554 YAP1 antibody [1A12]. Green : YAP1 Red : negative control FACS analysis of HeLa cells using GTX60554 YAP1 antibody [1A12]. Green : YAP1 Red : negative control](https://www.genetex.com/upload/website/prouct_img/normal/GTX60554/GTX60554_20170912_FACS_w_23061123_568.webp)
![IHC-P analysis of prostate cancer tissue using GTX60554 YAP1 antibody [1A12]. IHC-P analysis of prostate cancer tissue using GTX60554 YAP1 antibody [1A12].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60554/GTX60554_20170912_IHC-P_w_23061123_593.webp)
![IHC-P analysis of rectum cancer tissue using GTX60554 YAP1 antibody [1A12]. IHC-P analysis of rectum cancer tissue using GTX60554 YAP1 antibody [1A12].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60554/GTX60554_20170912_IHC-P_1_w_23061123_849.webp)
![ELISA analysis of antigen using GTX60554 YAP1 antibody [1A12].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng ELISA analysis of antigen using GTX60554 YAP1 antibody [1A12].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng](https://www.genetex.com/upload/website/prouct_img/normal/GTX60554/GTX60554_20170912_ELISA_w_23061123_672.webp)
![WB analysis of human YAP1 (AA: 250-447) recombinant protein using GTX60554 YAP1 antibody [1A12]. WB analysis of human YAP1 (AA: 250-447) recombinant protein using GTX60554 YAP1 antibody [1A12].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60554/GTX60554_20170912_WB_1_w_23061123_679.webp)
![YAP1 antibody immunoprecipitates YAP1 protein in IP experiments. IP samples: HeLa whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of YAP1 protein by 4 μg YAP1 antibody (GTX129151) 7.5 % SDS-PAGE The immunoprecipitated YAP1 protein was detected by YAP1 antibody (GTX129151) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]](https://www.genetex.com/upload/website/prouct_img/normal/GTX129151/GTX129151_41500_IP_w_23060523_133.webp)



![Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with YAP1 antibody [GT256] (GTX633541) diluted at 1:500.](https://www.genetex.com/upload/website/prouct_img/normal/GTX633541/GTX633541_42520_20160922_WB_shRNA_watermark_w_23061202_151.webp)
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with YAP1 antibody [HL1894] (GTX637643) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637643/GTX637643_T-44837_20221111_WB_22110919_573.webp)
