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Western Blot Positive WB detected in: 293 whole cell lysate, MCF-7 whole cell lysate, Rat Heart tissue All lanes: ACTN2 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 104 kDa Observed band size: 104 kDa
Western Blot Positive WB detected in: 293 whole cell lysate, MCF-7 whole cell lysate, Rat Heart tissue All lanes: ACTN2 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 104 kDa Observed band size: 104 kDa
Western Blot Positive WB detected in: 293 whole cell lysate, MCF-7 whole cell lysate, Rat Heart tissue All lanes: ACTN2 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 104 kDa Observed band size: 104 kDa

ACTN2 Antibody

CSB-PA001242LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetACTN2
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Overview

  • Supplier
    Cusabio
  • Product Name
    ACTN2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID88
  • Target name
    ACTN2
  • Target description
    actinin alpha 2
  • Target synonyms
    alpha-actinin skeletal muscle; alpha-actinin-2; CMD1AA; CMH23; F-actin cross-linking protein; MPD6; MYOCOZ; truncated actinin alpha 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35609
  • Protein Name
    Alpha-actinin-2
  • Scientific Description
    F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ACTN2 using anti-ACTN2 antibody (A03673-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: rat skeletal muscle tissue lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse skeletal muscle tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTN2 antigen affinity purified polyclonal antibody (Catalog # A03673-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACTN2 at approximately 104 kDa. The expected band size for ACTN2 is at 104 kDa.
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