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Anti-ACTN2 Antibody

Anti-ACTN2 Antibody

A38104
Antibodies.com
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-ACTN2 Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.8 mg/ml
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Scientific Description
    Rabbit polyclonal antibody to ACTN2
  • Reactivity
    Human, Mouse
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ACTN2 using anti-ACTN2 antibody (A03673-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: rat skeletal muscle tissue lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse skeletal muscle tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTN2 antigen affinity purified polyclonal antibody (Catalog # A03673-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACTN2 at approximately 104 kDa. The expected band size for ACTN2 is at 104 kDa.
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Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N1N3] (GTX103219) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Western Blot Positive WB detected in: 293 whole cell lysate, MCF-7 whole cell lysate, Rat Heart tissue All lanes: ACTN2 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 104 kDa Observed band size: 104 kDa
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