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Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Sarcomeric Alpha Actinin) Polyclonal Antibody, Unconjugated (bs-10367R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Sarcomeric Alpha Actinin) Polyclonal Antibody, Unconjugated (bs-10367R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Sarcomeric Alpha Actinin) Polyclonal Antibody, Unconjugated (bs-10367R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Sarcomeric Alpha Actinin Polyclonal Antibody

BS-10367R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetACTN2
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Overview

  • Supplier
    Bioss
  • Product Name
    Sarcomeric Alpha Actinin Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID88
  • Target name
    ACTN2
  • Target description
    actinin alpha 2
  • Target synonyms
    CMD1AA, CMH23, CMYO8, CMYP8, MPD6, MYOCOZ, alpha-actinin-2, F-actin cross-linking protein, alpha-actinin skeletal muscle
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35609
  • Protein Name
    Alpha-actinin-2
  • Reactivity
    Bovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

References

  • Ghrelin protects the myocardium with hypoxia/reoxygenation treatment through upregulating the expression of growth hormone, growth hormone secretagogue receptor and insulin-like growth factor-1, and promoting the phosphorylation of protein kinase B. Liu Y et al., 2018 Dec, Int J Mol Med
    Read this paper
  • Composite poly(lactic acid)/chitosan nanofibrous scaffolds for cardiac tissue engineering. Liu Y et al., 2017 Oct, Int J Biol Macromol
    Read this paper

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