ICC/IF analysis of COS7 cells transiently transfected with AKT3 plasmid using GTX84906 AKT3 antibody [9B2].
![IHC-P analysis of human lung tissue using GTX84906 AKT3 antibody [9B2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84906/GTX84906_3238_IHC-P_w_23061420_600.webp "IHC-P analysis of human lung tissue using GTX84906 AKT3 antibody [9B2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![WB analysis of SVT2 and PC12 cell lysate using GTX84906 AKT3 antibody [9B2]. Loading : 10 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX84906/GTX84906_3586_WB_w_23061420_339.webp "WB analysis of SVT2 and PC12 cell lysate using GTX84906 AKT3 antibody [9B2]. Loading : 10 ug per lane Dilution : 1:200")
![WB analysis of HEK293T cells transfected with AKT3 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84906 AKT3 antibody [9B2]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84906/GTX84906_4707_WB_w_23061420_130.webp "WB analysis of HEK293T cells transfected with AKT3 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84906 AKT3 antibody [9B2]. Loading : 5 ug per lane")
![WB analysis of BALB/C 3T3 cell lysate using GTX84906 AKT3 antibody [9B2]. Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX84906/GTX84906_4708_WB_w_23061420_283.webp "WB analysis of BALB/C 3T3 cell lysate using GTX84906 AKT3 antibody [9B2]. Dilution : 1:500")
![FACS analysis of HEK293T cells transfected with either AKT3 plasmid(Red) or empty vector control plasmid(Blue) using GTX84906 AKT3 antibody [9B2].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84906/GTX84906_611_FACS_w_23061420_968.webp "FACS analysis of HEK293T cells transfected with either AKT3 plasmid(Red) or empty vector control plasmid(Blue) using GTX84906 AKT3 antibody [9B2].")
ICC/IF analysis of COS7 cells transiently transfected with AKT3 plasmid using GTX84906 AKT3 antibody [9B2].
AKT3 antibody [9B2]
GTX84906
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetAKT3
Overview
- SupplierGeneTex
- Product NameAKT3 antibody [9B2] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500. ICC/IF: 1:100. IHC-P: 1:50. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID9B2
- Concentration0.25 mg/ml
- ConjugateUnconjugated
- Gene ID10000
- Target nameAKT3
- Target descriptionAKT serine/threonine kinase 3
- Target synonymsMPPH; MPPH2; PKB gamma; PKBG; PKB-GAMMA; PRKBG; RAC-gamma; RAC-gamma serine/threonine protein kinase; RAC-gamma serine/threonine-protein kinase; RAC-PK-gamma; STK-2; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
- HostMouse
- IsotypeIgG3
- Protein IDQ9Y243
- Protein NameRAC-gamma serine/threonine-protein kinase
- Scientific DescriptionIGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203