IHC-P analysis of human kidney tissue using GTX83663 alpha 1 Antitrypsin antibody [11G2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
![IHC-P analysis of human liver tissue using GTX83663 alpha 1 Antitrypsin antibody [11G2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83663/GTX83663_1678_IHC-P_w_23061420_895.webp "IHC-P analysis of human liver tissue using GTX83663 alpha 1 Antitrypsin antibody [11G2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human pancreas tissue using GTX83663 alpha 1 Antitrypsin antibody [11G2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83663/GTX83663_1679_IHC-P_w_23061420_192.webp "IHC-P analysis of human pancreas tissue using GTX83663 alpha 1 Antitrypsin antibody [11G2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IP analysis of DDDDK tagged alpha 1 Antitrypsin overexpressed HEK293T lysate using GTX83663 alpha 1 Antitrypsin antibody [11G2].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate](https://www.genetex.com/upload/website/prouct_img/normal/GTX83663/GTX83663_3328_IP_w_23061420_112.webp "IP analysis of DDDDK tagged alpha 1 Antitrypsin overexpressed HEK293T lysate using GTX83663 alpha 1 Antitrypsin antibody [11G2].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate")
![WB analysis of HEK293T cells transfected with alpha 1 Antitrypsin plasmid (Right) or empty vector (Left) for 48 hrs using GTX83663 alpha 1 Antitrypsin antibody [11G2]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX83663/GTX83663_3801_WB_w_23061420_366.webp "WB analysis of HEK293T cells transfected with alpha 1 Antitrypsin plasmid (Right) or empty vector (Left) for 48 hrs using GTX83663 alpha 1 Antitrypsin antibody [11G2]. Loading : 5 ug per lane")
![WB analysis of various cell lines using GTX83663 alpha 1 Antitrypsin antibody [11G2]. Loading : 35 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX83663/GTX83663_3803_WB_w_23061420_631.webp "WB analysis of various cell lines using GTX83663 alpha 1 Antitrypsin antibody [11G2]. Loading : 35 ug per lane")
![WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon) using GTX83663 alpha 1 Antitrypsin antibody [11G2]. Loading : 10 ug per lane Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX83663/GTX83663_4394_WB_w_23061420_903.webp "WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon) using GTX83663 alpha 1 Antitrypsin antibody [11G2]. Loading : 10 ug per lane Dilution : 1:500")
IHC-P analysis of human kidney tissue using GTX83663 alpha 1 Antitrypsin antibody [11G2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
alpha 1 Antitrypsin antibody [11G2]
GTX83663
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Human, Monkey
TargetSERPINA1
Overview
- SupplierGeneTex
- Product Namealpha 1 Antitrypsin antibody [11G2] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:20000-1:40000. IHC-P: 1:150. IP: 2ug/500ul. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID11G2
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5265
- Target nameSERPINA1
- Target descriptionserpin family A member 1
- Target synonymsA1A; A1AT; AAT; alpha-1 antitrypsin; alpha-1 protease inhibitor; alpha-1 proteinase inhibitor; alpha-1-antiproteinase; alpha-1-antitrypsin; alpha-1-antitrypsin short transcript variant 1C4; alpha-1-antitrypsin short transcript variant 1C5; alpha1AT; anti-elastase; epididymis secretory sperm binding protein; nNIF; PI; PI1; PRO2275; protease inhibitor 1 (anti-elastase), alpha-1-antitrypsin; serine (or cysteine) proteinase inhibitor, clade A, member 1; serpin A1; serpin peptidase inhibitor clade A (alpha-1antiproteinase, antitrypsin) member 1; serpin peptidase inhibitor clade A member 1; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
- HostMouse
- IsotypeIgG1
- Protein IDP01009
- Protein NameAlpha-1-antitrypsin
- Scientific DescriptionInhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Short peptide from AAT (SPAAT) is a reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
- ReactivityCanine, Human, Monkey
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Evidence of acrolein in synovial fluid of dogs with osteoarthritis as a potential inflammatory biomarker. Herr SA et al., 2021 Oct 20, BMC Musculoskelet DisordRead more