![IHC-P analysis of human thyroid carcinoma tissue using GTX83666 alpha 1 Antitrypsin antibody [9A1]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. IHC-P analysis of human thyroid carcinoma tissue using GTX83666 alpha 1 Antitrypsin antibody [9A1]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83666/GTX83666_1684_IHC-P_w_23061420_260.webp)
IHC-P analysis of human thyroid carcinoma tissue using GTX83666 alpha 1 Antitrypsin antibody [9A1]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
alpha 1 Antitrypsin antibody [9A1]
GTX83666
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Ferret, Human, Monkey
TargetSERPINA1
Overview
- SupplierGeneTex
- Product Namealpha 1 Antitrypsin antibody [9A1]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:1000. IHC-P: 1:150. FACS: 1:100. IP: 2ug/500ul. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID9A1
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5265
- Target nameSERPINA1
- Target descriptionserpin family A member 1
- Target synonymsA1A, A1AT, AAT, PI, PI1, PRO2275, alpha1AT, nNIF, alpha-1-antitrypsin, alpha-1 antitrypsin, alpha-1 protease inhibitor, alpha-1 proteinase inhibitor, alpha-1-antiproteinase, anti-elastase, epididymis secretory sperm binding protein, protease inhibitor 1 (anti-elastase), alpha-1-antitrypsin, serine (or cysteine) proteinase inhibitor, clade A, member 1, serpin A1, serpin peptidase inhibitor clade A (alpha-1antiproteinase, antitrypsin) member 1, serpin peptidase inhibitor clade A member 1, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
- HostMouse
- IsotypeIgG1
- Protein IDP01009
- Protein NameAlpha-1-antitrypsin
- Scientific DescriptionInhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Short peptide from AAT (SPAAT) is a reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
- ReactivityCanine, Ferret, Human, Monkey
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- He N, Liu X, Vegter AR, et al. Ferret models of alpha-1 antitrypsin deficiency develop lung and liver disease. JCI Insight. 2022,7(5). doi: 10.1172/jci.insight.143004Read this paper
![IHC-P analysis of human liver tissue using GTX83666 alpha 1 Antitrypsin antibody [9A1]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. IHC-P analysis of human liver tissue using GTX83666 alpha 1 Antitrypsin antibody [9A1]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83666/GTX83666_1685_IHC-P_w_23061420_407.webp)
![WB analysis of HEK293T cells transfected with alpha 1 Antitrypsin plasmid (Right) or empty vector (Left) for 48 hrs using GTX83666 alpha 1 Antitrypsin antibody [9A1]. Loading : 5 ug per lane WB analysis of HEK293T cells transfected with alpha 1 Antitrypsin plasmid (Right) or empty vector (Left) for 48 hrs using GTX83666 alpha 1 Antitrypsin antibody [9A1]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX83666/GTX83666_3806_WB_w_23061420_414.webp)
![IP analysis of DDDDK tagged alpha 1 Antitrypsin overexpressed HEK293T lysate using GTX83666 alpha 1 Antitrypsin antibody [9A1].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2μg antibody / 500μl cell lysate IP analysis of DDDDK tagged alpha 1 Antitrypsin overexpressed HEK293T lysate using GTX83666 alpha 1 Antitrypsin antibody [9A1].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2μg antibody / 500μl cell lysate](https://www.genetex.com/upload/website/prouct_img/normal/GTX83666/GTX83666_3330_IP_w_23061420_292.webp)
![IHC-P analysis of human liver carcinoma tissue using GTX83666 alpha 1 Antitrypsin antibody [9A1]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. IHC-P analysis of human liver carcinoma tissue using GTX83666 alpha 1 Antitrypsin antibody [9A1]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83666/GTX83666_1683_IHC-P_w_23061420_288.webp)
![WB analysis of various cell lines using GTX83666 alpha 1 Antitrypsin antibody [9A1]. Loading : 35 ug per lane WB analysis of various cell lines using GTX83666 alpha 1 Antitrypsin antibody [9A1]. Loading : 35 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX83666/GTX83666_3808_WB_w_23061420_787.webp)
![WB analysis of human tissues (Lane 1-Uterus ; Lane 2-Breast ; Lane 3-Brain ; Lane 4-Liver ; Lane 5-Ovary ; Lane 6-Thyroid gland ; Lane 7-colon) using GTX83666 alpha 1 Antitrypsin antibody [9A1]. Loading : 10 ug per lane Dilution : 1:200 WB analysis of human tissues (Lane 1-Uterus ; Lane 2-Breast ; Lane 3-Brain ; Lane 4-Liver ; Lane 5-Ovary ; Lane 6-Thyroid gland ; Lane 7-colon) using GTX83666 alpha 1 Antitrypsin antibody [9A1]. Loading : 10 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX83666/GTX83666_3807_WB_w_23061420_687.webp)
![IHC-P analysis of human pancreas tissue using GTX83666 alpha 1 Antitrypsin antibody [9A1]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. IHC-P analysis of human pancreas tissue using GTX83666 alpha 1 Antitrypsin antibody [9A1]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83666/GTX83666_1686_IHC-P_w_23061420_788.webp)
![IHC-P analysis of human endometrium adenocarcinoma tissue using GTX83666 alpha 1 Antitrypsin antibody [9A1]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. IHC-P analysis of human endometrium adenocarcinoma tissue using GTX83666 alpha 1 Antitrypsin antibody [9A1]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83666/GTX83666_1682_IHC-P_w_23061420_370.webp)
![FACS analysis of HEK293T cells transfected with either alpha 1 Antitrypsin plasmid(Red) or empty vector control plasmid(Blue) using GTX83666 alpha 1 Antitrypsin antibody [9A1]. FACS analysis of HEK293T cells transfected with either alpha 1 Antitrypsin plasmid(Red) or empty vector control plasmid(Blue) using GTX83666 alpha 1 Antitrypsin antibody [9A1].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83666/GTX83666_108_FACS_w_23061420_788.webp)

![alpha 1 antitrypsin antibody [N1C2] detects alpha 1 antitrypsin protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. alpha 1 antitrypsin antibody [N1C2] (GTX112707) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX112707/GTX112707_40492_20151030_IHC-P_2_w_23060500_985.webp)
![IHC-P analysis of human liver tissue using GTX83663 alpha 1 Antitrypsin antibody [11G2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83663/GTX83663_1678_IHC-P_w_23061420_895.webp)
![IHC-P analysis of human liver tissue using GTX83665 alpha 1 Antitrypsin antibody [15H10]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83665/GTX83665_1680_IHC-P_w_23061420_746.webp)




![FACS analysis of 70% ethanol fixed HepG2 cells using GTX15777 alpha 1 Antitrypsin antibody [TMF1#4B5]. Red histogram : Primary antibody Pink histogram : mouse isotype control Fixation : 70% ethanol for 10 minutes Permeabilization : 0.25% Trito X-100 for 20 minutes Dilution : 3-5 μg/million cells in 2.5% BSA for 30 minutes at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX15777/GTX15777_116_FACS_w_23060620_281.webp)