IHC-P analysis of human colon carcinoma tissue using GTX15648 alpha Actinin 4 antibody [7H6]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
![IHC-P analysis of human kidney tissue using GTX15648 alpha Actinin 4 antibody [7H6]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX15648/GTX15648_1025_IHC-P_w_23060620_844.webp "IHC-P analysis of human kidney tissue using GTX15648 alpha Actinin 4 antibody [7H6]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200")
![IHC-P analysis of human tonsil tissue using GTX15648 alpha Actinin 4 antibody [7H6]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX15648/GTX15648_1026_IHC-P_w_23060620_255.webp "IHC-P analysis of human tonsil tissue using GTX15648 alpha Actinin 4 antibody [7H6]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200")
![WB analysis of samples using GTX15648 alpha Actinin 4 antibody [7H6].](https://www.genetex.com/upload/website/prouct_img/normal/GTX15648/GTX15648_2094_WB_w_23060620_562.webp "WB analysis of samples using GTX15648 alpha Actinin 4 antibody [7H6].")
IHC-P analysis of human colon carcinoma tissue using GTX15648 alpha Actinin 4 antibody [7H6]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
alpha Actinin 4 antibody [7H6]
GTX15648
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetACTN4
Overview
- SupplierGeneTex
- Product Namealpha Actinin 4 antibody [7H6]
- Delivery Days Customer9
- Application Supplier NoteWB: 2 microg/mL. ICC/IF: 4 microg/mL. IHC-P: 4 microg/ml. FACS: 1microg per 106 cells. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID7H6
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID81
- Target nameACTN4
- Target descriptionactinin alpha 4
- Target synonymsACTININ-4; alpha-actinin-4; focal segmental glomerulosclerosis 1; FSGS; FSGS1; non-muscle alpha-actinin 4
- HostMouse
- IsotypeIgG
- Protein IDO43707
- Protein NameAlpha-actinin-4
- Scientific DescriptionAlpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- ACTN4 Mediates SEPT14 Mutation-Induced Sperm Head Defects. Lin YH et al., 2020 Nov 19, BiomedicinesRead more
- Characterisation of adipocyte-derived extracellular vesicle subtypes identifies distinct protein and lipid signatures for large and small extracellular vesicles. Durcin M et al., 2017, J Extracell VesiclesRead more