Anterior Gradient 2 antibody [GT5812] detects secreted Anterior Gradient 2 protein by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Anterior Gradient 2 stained by Anterior Gradient 2 antibody [GT5812] (GTX634163) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
![Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Anterior Gradient 2 antibody [GT5812] (GTX634163) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX634163/GTX634163_42849_20170511_WB_w_23061202_819.webp "Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Anterior Gradient 2 antibody [GT5812] (GTX634163) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.")
![Anterior Gradient 2 antibody [GT5812] detects secreted Anterior Gradient 2 protein by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Anterior Gradient 2 stained by Anterior Gradient 2 antibody [GT5812] (GTX634163) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX634163/GTX634163_42849_20180504_IHC-P_1_w_23061202_464.webp "Anterior Gradient 2 antibody [GT5812] detects secreted Anterior Gradient 2 protein by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Anterior Gradient 2 stained by Anterior Gradient 2 antibody [GT5812] (GTX634163) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Anterior Gradient 2 antibody [GT5812] detects Anterior Gradient 2 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Anterior Gradient 2 stained by Anterior Gradient 2 antibody [GT5812] (GTX634163) diluted at 1:500. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX634163/GTX634163_42849_20180523_ICC_IF_w_23061202_994.webp "Anterior Gradient 2 antibody [GT5812] detects Anterior Gradient 2 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Anterior Gradient 2 stained by Anterior Gradient 2 antibody [GT5812] (GTX634163) diluted at 1:500. Blue: Hoechst 33342 staining.")
Anterior Gradient 2 antibody [GT5812] detects secreted Anterior Gradient 2 protein by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Anterior Gradient 2 stained by Anterior Gradient 2 antibody [GT5812] (GTX634163) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Anterior Gradient 2 antibody [GT5812]
GTX634163
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetAGR2
Overview
- SupplierGeneTex
- Product NameAnterior Gradient 2 antibody [GT5812]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT5812
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID10551
- Target nameAGR2
- Target descriptionanterior gradient 2, protein disulphide isomerase family member
- Target synonymsAG-2, AG2, GOB-4, HAG-2, HEL-S-116, HPC8, PDIA17, RIFTD, XAG-2, anterior gradient protein 2 homolog, anterior gradient homolog 2, epididymis secretory protein Li 116, protein disulfide isomerase family A, member 17, secreted cement gland homolog, secreted cement gland protein XAG-2 homolog
- HostMouse
- IsotypeIgG2b
- Protein IDO95994
- Protein NameAnterior gradient protein 2 homolog
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Wang D, Xu Q, Yuan Q, et al. Proteasome inhibition boosts autophagic degradation of ubiquitinated-AGR2 and enhances the antitumor efficiency of bevacizumab. Oncogene. 2019,38(18):3458-3474. doi: 10.1038/s41388-019-0675-zRead this paper
Datasheet
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