IHC analysis of ADH5 using anti-ADH5 antibody (RP1111). ADH5 was detected in paraffin-embedded section of human liver cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ADH5 Antibody (RP1111) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of ADH5 using anti-ADH5 antibody (RP1111). ADH5 was detected in paraffin-embedded section of human liver cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ADH5 Antibody (RP1111) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-Alcohol dehydrogenase class-3 ADH5 Antibody Picoband(r)
RP1111
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetADH5
Overview
- SupplierBoster Bio
- Product NameAnti-ADH5 Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID128
- Target nameADH5
- Target descriptionalcohol dehydrogenase 5 (class III), chi polypeptide
- Target synonymsADH-3; ADHX; alcohol dehydrogenase (class III), chi polypeptide; alcohol dehydrogenase class chi chain; alcohol dehydrogenase class-3; alcohol dehydrogenase class-III; AMEDS; BMFS7; epididymis secretory sperm binding protein Li 60p; FALDH; FDH; formaldehyde dehydrogenase; glutathione-dependent formaldehyde dehydrogenase; GSH-FDH; GSNOR; HEL-S-60p; S-(hydroxymethyl)glutathione dehydrogenase; S-nitrosoglutathione reductase
- HostRabbit
- IsotypeIgG
- Protein IDP11766
- Protein NameAlcohol dehydrogenase class-3
- Scientific DescriptionBoster Bio Anti-Alcohol dehydrogenase class-3 ADH5 Antibody catalog # RP1111. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203