Anti-Alpha-enolase [EnL5]

Absolute Antibody

Anti-Alpha-enolase [EnL5]

7

This antibody binds alpha-enolase, which is a glycolytic enzyme expressed in most tissues. It catalysis the conversion of 2-phosphoglycerate to phosphoenolpyruvate. In addition to glycolysis, it is involved in various processes such as growth control, hyp

The binding specificity of this antibody for alpha-enolase was done using ELISA. This antibody was also used for detection of alpha-enolase expressed in the PE089 cells using flow cytometry. This antibody was also used for immunofluorescent and immunocytochemical staining of alpha-enolase molecules expressed in PE089 cells (PMID: 20655599).

Flow Cytometry, ImmunoFluorescence, ELISA

ELISA; FC; IF

Research Use Only

Monoclonal

EnL5

ENO1

enolase 1

2-phospho-D-glycerate hydro-lyase; alpha enolase like 1; alpha-enolase; c-myc promoter-binding protein-1; ENO1L1; enolase 1, (alpha); enolase-alpha; epididymis secretory protein Li 17; HEL-S-17; MPB1; MYC promoter-binding protein 1; NNE; non-neural enolase; phosphopyruvate hydratase; plasminogen-binding protein; PPH; tau-crystallin

Rabbit

IgG

Alpha-enolase

This chimeric rabbit antibody was made using the variable domain sequences of the original Chicken IgG format for improved compatibility with existing reagents assays and techniques.

Human

Human

The original antibody was generated by immunizing female white leghorn (Gallus domesticus) chickens with 100 mug purified alfa-enolase in an equal volume of Freund's complete adjuvant by an intramuscular injection. The total RNA was extracted from the enlarged spleens for scFv antibody library construction. This clone was isolated by panning the scFv library against alpha-enolase.

-20°C,2°C to 8°C

12352203