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Immunohistochemical staining of human cerebral cortex shows neuropil and cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows neuropil and cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows neuropil and cytoplasmic positivity in neurons.

Anti-APBB1 Antibody

HPA038521
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetAPBB1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-APBB1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID322
  • Target name
    APBB1
  • Target description
    amyloid beta precursor protein binding family B member 1
  • Target synonyms
    adaptor protein FE65a2; amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65); amyloid-beta A4 precursor protein-binding family B member 1; FE65; MGC:9072; RIR; stat-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00213
  • Protein Name
    Amyloid-beta A4 precursor protein-binding family B member 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FE65 using anti-FE65 antibody (PB9684). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Brain Tissue Lysate at 50ug, Lane 3: HELA Whole Cell Lysate at 40ug, Lane 4: U87 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FE65 antigen affinity purified polyclonal antibody (Catalog # PB9684) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FE65 at approximately 65 kDa. The expected band size for FE65 is at 65 kDa.
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