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ProductsBack/APBB1 / FE65 Antibody

APBB1 / FE65 Antibody

LS-C335192
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetAPBB1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    APBB1 / FE65 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 49kDa/52kDa/53kDa/54kDa/76kDa/77kDa, while the observed MW by Western blot was 63kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IF (1:50 - 1:200), IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 49kDa/52kDa/53kDa/54kDa/76kDa/77kDa, while the observed MW by Western blot was 63kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID322
  • Target name
    APBB1
  • Target description
    amyloid beta precursor protein binding family B member 1
  • Target synonyms
    FE65, MGC:9072, RIR, amyloid beta precursor protein binding family B member 1, adaptor protein FE65a2, amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65), stat-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FE65 using anti-FE65 antibody (PB9684). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Brain Tissue Lysate at 50ug, Lane 3: HELA Whole Cell Lysate at 40ug, Lane 4: U87 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FE65 antigen affinity purified polyclonal antibody (Catalog # PB9684) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FE65 at approximately 65 kDa. The expected band size for FE65 is at 65 kDa.
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