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Immunohistochemical staining of human colon shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows strong granular cytoplasmic positivity in glandular cells.

Anti-C1QBP Antibody

HPA026483
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetC1QBP
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-C1QBP Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID708
  • Target name
    C1QBP
  • Target description
    complement C1q binding protein
  • Target synonyms
    COXPD33, GC1QBP, HABP1, SF2AP32, SF2p32, gC1Q-R, gC1qR, p32, complement component 1 Q subcomponent-binding protein, mitochondrial, ASF/SF2-associated protein p32, C1q globular domain-binding protein, complement component 1, q subcomponent binding protein, glycoprotein gC1qBP, hyaluronan-binding protein 1, mitochondrial matrix protein p32, p33, splicing factor SF2-associated protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ07021
  • Protein Name
    Complement component 1 Q subcomponent-binding protein, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of C1QBP using anti-C1QBP antibody (PB9921). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat spleen tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C1QBP antigen affinity purified polyclonal antibody (Catalog # PB9921) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C1QBP at approximately 35 kDa. The expected band size for C1QBP is at 31 kDa.
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