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GC1qR / C1QBP Antibody

LS-C331747
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetC1QBP
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GC1qR / C1QBP Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 31kDa, while the observed MW by Western blot was 36kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IF (1:50 - 1:200), IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 31kDa, while the observed MW by Western blot was 36kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID708
  • Target name
    C1QBP
  • Target description
    complement C1q binding protein
  • Target synonyms
    ASF/SF2-associated protein p32; C1q globular domain-binding protein; complement component 1 Q subcomponent-binding protein, mitochondrial; complement component 1, q subcomponent binding protein; COXPD33; GC1QBP; gC1qR; gC1Q-R; glycoprotein gC1qBP; HABP1; hyaluronan-binding protein 1; mitochondrial matrix protein p32; p32; p33; SF2AP32; SF2p32; splicing factor SF2-associated protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of C1QBP using anti-C1QBP antibody (PB9921). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat spleen tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C1QBP antigen affinity purified polyclonal antibody (Catalog # PB9921) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C1QBP at approximately 35 kDa. The expected band size for C1QBP is at 31 kDa.
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