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Western Blot analysis of various cells using C1QBP Polyclonal Antibody diluted at 1:1000
Western Blot analysis of various cells using C1QBP Polyclonal Antibody diluted at 1:1000
Western Blot analysis of various cells using C1QBP Polyclonal Antibody diluted at 1:1000

C1QBP Antibody

CSB-PA001089
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetC1QBP
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Overview

  • Supplier
    Cusabio
  • Product Name
    C1QBP Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID708
  • Target name
    C1QBP
  • Target description
    complement C1q binding protein
  • Target synonyms
    COXPD33, GC1QBP, HABP1, SF2AP32, SF2p32, gC1Q-R, gC1qR, p32, complement component 1 Q subcomponent-binding protein, mitochondrial, ASF/SF2-associated protein p32, C1q globular domain-binding protein, complement component 1, q subcomponent binding protein, glycoprotein gC1qBP, hyaluronan-binding protein 1, mitochondrial matrix protein p32, p33, splicing factor SF2-associated protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ07021
  • Protein Name
    Complement component 1 Q subcomponent-binding protein, mitochondrial
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with C1qBP antibody [GT1250] (GTX02847) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Figure 1. Western blot analysis of C1QBP using anti-C1QBP antibody (PB9921). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat spleen tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C1QBP antigen affinity purified polyclonal antibody (Catalog # PB9921) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C1QBP at approximately 35 kDa. The expected band size for C1QBP is at 31 kDa.
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