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Anti-CBX1 [RAB-C145]

Ab01704-1.1
Absolute Antibody
ApplicationsImmunoFluorescence, ImmunoPrecipitation, ChIP Chromatin ImmunoPrecipitation, ELISA
Product group Antibodies
TargetCBX1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CBX1 [RAB-C145]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody has been used and recommended for the following methods: ELISA; IF; IP-MS; ChIP.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, ChIP Chromatin ImmunoPrecipitation, ELISA
  • Applications Supplier
    ELISA; IF; IP-MS; ChIP
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RAB-C145
  • Gene ID10951
  • Target name
    CBX1
  • Target description
    chromobox 1
  • Target synonyms
    CBX, HP1-BETA, HP1Hs-beta, HP1Hsbeta, Hp1beta, M31, MOD1, p25beta, chromobox protein homolog 1, HP1 beta homolog, chromobox homolog 1 (HP1 beta homolog Drosophila ), heterochromatin protein 1 homolog beta, heterochromatin protein 1-beta, heterochromatin protein p25 beta, modifier 1 protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP83916
  • Protein Name
    Chromobox protein homolog 1
  • Scientific Description
    This chimeric mouse antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    This antibody was obtained by recombinant antibody (rAb) phage display recognizing CBX1 protein under non-denaturing conditions.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CBX1/HP1 beta using anti-CBX1/HP1 beta antibody (A04206-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: huamn SW620 whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBX1/HP1 beta antigen affinity purified polyclonal antibody (Catalog # A04206-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CBX1/HP1 beta at approximately 21 kDa. The expected band size for CBX1/HP1 beta is at 21 kDa.
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