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ProductsBack/Goat anti-CBX1 / HP1-Beta

Goat anti-CBX1 / HP1-Beta

EB06958
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCBX1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-CBX1 / HP1-Beta Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID10951
  • Target name
    CBX1
  • Target description
    chromobox 1
  • Target synonyms
    CBX; chromobox homolog 1 (HP1 beta homolog Drosophila ); chromobox protein homolog 1; heterochromatin protein 1 homolog beta; heterochromatin protein 1-beta; heterochromatin protein p25 beta; HP1 beta homolog; HP1-BETA; HP1Hsbeta; HP1Hs-beta; M31; MOD1; modifier 1 protein; p25beta
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_006798.1; NP_001120700.1. GeneIDs all Nonhuman: 12412 (mouse); 360609 (rat);. Purification: Antigen affinity purified. Names and symbols: CBX1; HP1-BETA; chromobox homolog 1 (HP1 beta homolog Drosophila ); HGNC:1551; CBX; HP1Hs-beta; M31; MOD1; chromobox homolog 1 (Drosophila HP1 beta); heterochromatin protein p25 beta; HP1Hsbeta; p25beta; heterochromatin protein 1-beta; modifier 1 protein
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CBX1/HP1 beta using anti-CBX1/HP1 beta antibody (A04206-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: huamn SW620 whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBX1/HP1 beta antigen affinity purified polyclonal antibody (Catalog # A04206-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CBX1/HP1 beta at approximately 21 kDa. The expected band size for CBX1/HP1 beta is at 21 kDa.
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