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Anti-HP1alpha, beta and gamma Polyclonal Antibody

Anti-HP1alpha, beta and gamma Polyclonal Antibody

25292
BPS Bioscience
ApplicationsChIP Chromatin ImmunoPrecipitation
Product group Antibodies
TargetCBX1
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Overview

  • Supplier
    BPS Bioscience
  • Product Name
    Anti-HP1alpha, beta and gamma Polyclonal Antibody
  • Delivery Days Customer
    7
  • Applications
    ChIP Chromatin ImmunoPrecipitation
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID10951
  • Target name
    CBX1
  • Target description
    chromobox 1
  • Target synonyms
    CBX, HP1-BETA, HP1Hs-beta, HP1Hsbeta, Hp1beta, M31, MOD1, p25beta, chromobox protein homolog 1, HP1 beta homolog, chromobox homolog 1 (HP1 beta homolog Drosophila ), heterochromatin protein 1 homolog beta, heterochromatin protein 1-beta, heterochromatin protein p25 beta, modifier 1 protein
  • Protein IDP45973
  • Protein Name
    Chromobox protein homolog 5
  • Scientific Description
    Polyclonal antibody raised in rabbit against human HP1beta (Heterochromatin protein 1 homolog beta), using the full length recombinant GST tagged protein. The antibody also recognizes the HP1alpha and HP1gamma isoforms.
  • Reactivity Supplier
    Human, Mouse
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CBX1/HP1 beta using anti-CBX1/HP1 beta antibody (A04206-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: huamn SW620 whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBX1/HP1 beta antigen affinity purified polyclonal antibody (Catalog # A04206-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CBX1/HP1 beta at approximately 21 kDa. The expected band size for CBX1/HP1 beta is at 21 kDa.
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