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Figure 1. Immunohistochemistry validation of SEMA7A using Anti-CD108 SEMA7A Antibody (A03832-1). Immunohistochemical analysis of paraffin-embedded human-brain
Figure 1. Immunohistochemistry validation of SEMA7A using Anti-CD108 SEMA7A Antibody (A03832-1). Immunohistochemical analysis of paraffin-embedded human-brain
Figure 1. Immunohistochemistry validation of SEMA7A using Anti-CD108 SEMA7A Antibody (A03832-1). Immunohistochemical analysis of paraffin-embedded human-brain

Anti-CD108 SEMA7A Antibody

A03832-1
Boster Bio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSEMA7A
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-CD108 SEMA7A Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Gene ID8482
  • Target name
    SEMA7A
  • Target description
    semaphorin 7A (JohnMiltonHagen blood group)
  • Target synonyms
    CD108, CDw108, H-SEMA-K1, H-Sema-L, JMH, PFIC11, SEMAK1, SEMAL, semaphorin-7A, JMH blood group antigen, John Milton Hagen blood group H-Sema K1, john-Milton-Hargen human blood group Ag, sema K1, sema L, sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (JMH blood group), sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, 7A, semaphorin 7A (John Milton Hagen blood group), semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group), semaphorin-K1, semaphorin-L
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75326
  • Protein Name
    Semaphorin-7A
  • Scientific Description
    Boster Bio Anti-CD108 SEMA7A Antibody catalog # A03832-1. Tested in IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Semaphorin 7a/SEMA7A using anti-Semaphorin 7a/SEMA7A antibody (A03832-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Semaphorin 7a/SEMA7A antigen affinity purified polyclonal antibody (Catalog # A03832-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Semaphorin 7a/SEMA7A at approximately 75 kDa. The expected band size for Semaphorin 7a/SEMA7A is at 75 kDa.
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