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Rat spleen cells probed with Rabbit Anti-CD40L Polyclonal Antibody (bs-1286R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary Antibody.
Rat spleen cells probed with Rabbit Anti-CD40L Polyclonal Antibody (bs-1286R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary Antibody.
Rat spleen cells probed with Rabbit Anti-CD40L Polyclonal Antibody (bs-1286R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary Antibody.

CD40L Polyclonal Antibody

BS-1286R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCD40LG
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Overview

  • Supplier
    Bioss
  • Product Name
    CD40L Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID959
  • Target name
    CD40LG
  • Target description
    CD40 ligand
  • Target synonyms
    CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L, CD40 ligand, CD40 antigen ligand, CD40-L, T-B cell-activating molecule, T-cell antigen Gp39, TNF-related activation protein, tumor necrosis factor (ligand) superfamily member 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29965
  • Protein Name
    CD40 ligand
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Wang B, Feng C, Dang J, et al. Anti-Adhesive, Platelet Gathering Effects of c-RGD Modified Poly(p-dioxanone-co-l-Phe) Electrospun Membrane and Its Comprehensive Application in Intestinal Anastomosis. Macromol Biosci. 2020,20(2):e1900344. doi: 10.1002/mabi.201900344
    Read this paper
  • Zhang F, Song SS, Shu JL, et al. BAFF upregulates CD28/B7 and CD40/CD154 expression and promotes mouse T and B cell interaction in vitro via BAFF receptor. Acta Pharmacol Sin. 2016,37(8):1101-9. doi: 10.1038/aps.2016.15
    Read this paper
  • Rui-Kai Z, Jian L. Effects of xinfeng capsules on expression of platelet granule membrane protein 140 and platelet cluster of differentiation 40 ligand in peripheral blood of adjuvant arthritis rats. Int J Rheumatol. 2012,2012:139696. doi: 10.1155/2012/139696
    Read this paper

Datasheet

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Figure 1. Western blot analysis of CD40L using anti-CD40L antibody (A01114-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD40L antigen affinity purified polyclonal antibody (Catalog # A01114-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD40L at approximately 19KD. The expected band size for CD40L is at 29KD.
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