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ProductsBack/CD40L / CD154 antibody [5C8]

CD40L / CD154 antibody [5C8]

GTX01484
GeneTex
ApplicationsFlow Cytometry
DownloadDatasheet
Product group Antibodies
TargetCD40LG
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD40L / CD154 antibody [5C8]
  • Delivery Days Customer
    9
  • Application Supplier Note
    *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5C8
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID959
  • Target name
    CD40LG
  • Target description
    CD40 ligand
  • Target synonyms
    CD154; CD40 antigen ligand; CD40 ligand; CD40L; CD40-L; gp39; hCD40L; HIGM1; IGM; IMD3; T-B cell-activating molecule; T-BAM; T-cell antigen Gp39; TNF-related activation protein; TNFSF5; TRAP; tumor necrosis factor (ligand) superfamily member 5
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP29965
  • Protein Name
    CD40 ligand
  • Scientific Description
    The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • Candida-Reactive T Cells for the Diagnosis of Invasive Candida Infection-A Prospective Pilot Study. Koehler FC et al., 2018, Front Microbiol
    Read more
  • Functional characterization of T-cells from palatine tonsils in patients with chronic tonsillitis. Geibetaler K et al., 2017, PLoS One
    Read more
  • Highly Predictive Model for a Protective Immune Response to the A(H1N1)pdm2009 Influenza Strain after Seasonal Vaccination. Juerchott K et al., 2016, PLoS One
    Read more
  • Effects of aging on human leukocytes (part II): immunophenotyping of adaptive immune B and T cell subsets. Stervbo U et al., 2015 Oct, Age (Dordr)
    Read more
  • Analysis of the functional WT1-specific T-cell repertoire in healthy donors reveals a discrepancy between CD4(+) and CD8(+) memory formation. Schmied S et al., 2015 Aug, Immunology
    Read more

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Figure 1. Western blot analysis of CD40L using anti-CD40L antibody (A01114-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD40L antigen affinity purified polyclonal antibody (Catalog # A01114-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD40L at approximately 19KD. The expected band size for CD40L is at 29KD.
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