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ProductsBack/anti-CD49a / Integrin alpha 1 antibody [TS2/7]

anti-CD49a / Integrin alpha 1 antibody [TS2/7]

ARG22420
Arigo Biolaboratories
ApplicationsFlow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Other Application
Product group Antibodies
ReactivityHuman
TargetITGA1
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Overview

  • Supplier
    Arigo Biolaboratories
  • Product Name
    anti-CD49a / Integrin alpha 1 antibody [TS2/7]
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    TS2/7
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3672
  • Target name
    ITGA1
  • Target description
    integrin subunit alpha 1
  • Target synonyms
    CD49a, VLA1, integrin alpha-1, CD49 antigen-like family member A, VLA-1, laminin and collagen receptor, very late activation protein 1, very late antigen-1
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    This gene encodes the alpha 1 subunit of integrin receptors. This protein heterodimerizes with the beta 1 subunit to form a cell-surface receptor for collagen and laminin. The heterodimeric receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. The alpha 1 subunit contains an inserted (I) von Willebrand factor type I domain which is thought to be involved in collagen binding. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Integrin Alpha 1/ITGA1 using anti-Integrin Alpha 1/ITGA1 antibody (PA1045-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin Alpha 1/ITGA1 antigen affinity purified polyclonal antibody (Catalog # PA1045-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin Alpha 1/ITGA1 at approximately 190-200 kDa. The expected band size for Integrin Alpha 1/ITGA1 is at 190-200 kDa.
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