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ProductsBack/Anti-CD81 antigen CD81 Antibody
Western Blot (WB) analysis of 4T1 cells using CD81 Polyclonal antibody.
Western Blot (WB) analysis of 4T1 cells using CD81 Polyclonal antibody.
Western Blot (WB) analysis of 4T1 cells using CD81 Polyclonal antibody.

Anti-CD81 antigen CD81 Antibody

A01281-1
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCD81
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-CD81 antigen CD81 Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Concentration
    1 mg/ml
  • Gene ID975
  • Target name
    CD81
  • Target description
    CD81 molecule
  • Target synonyms
    CVID6, S5.7, TAPA1, TSPAN28, CD81 antigen, 26 kDa cell surface protein TAPA-1, CD81 antigen (target of antiproliferative antibody 1), tetraspanin-28, tspan-28
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP60033
  • Protein Name
    CD81 antigen
  • Scientific Description
    Boster Bio Anti-CD81 antigen CD81 Antibody catalog # A01281-1. Tested in IF, WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Cao G, Meng X, Han X, et al. Exosomes derived from circRNA Rtn4-modified BMSCs attenuate TNF-α-induced cytotoxicity and apoptosis in murine MC3T3-E1 cells by sponging miR-146a. Biosci Rep. 2020,40(5). doi: 10.1042/BSR20193436
    Read this paper
  • Qu Q, Pang Y, Zhang C, et al. Exosomes derived from human umbilical cord mesenchymal stem cells inhibit vein graft intimal hyperplasia and accelerate reendothelialization by enhancing endothelial function. Stem Cell Res Ther. 2020,11(1):133. doi: 10.1186/s13287-020-01639-1
    Read this paper
  • Liu X, Lu Y, Xu Y, et al. Exosomal transfer of miR-501 confers doxorubicin resistance and tumorigenesis via targeting of BLID in gastric cancer. Cancer Lett. 2019,459:122-134. doi: 10.1016/j.canlet.2019.05.035
    Read this paper

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Figure 1. Western blot analysis of TAPA1 using anti-TAPA1 antibody (A01281-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse raw264.7 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAPA1 antigen affinity purified polyclonal antibody (Catalog # A01281-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAPA1 at approximately 22KD. The expected band size for TAPA1 is at 22KD.
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