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Immunohistochemical staining of human caudate nucleus shows strong cytoplasmic positivity in a large neurons.
Immunohistochemical staining of human caudate nucleus shows strong cytoplasmic positivity in a large neurons.
Immunohistochemical staining of human caudate nucleus shows strong cytoplasmic positivity in a large neurons.

Anti-CHAT Antibody

HPA048547
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetCHAT
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CHAT
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1103
  • Target name
    CHAT
  • Target description
    choline O-acetyltransferase
  • Target synonyms
    acetyl CoA:choline O-acetyltransferase; CHOACTASE; choline acetylase; choline O-acetyltransferase; CMS1A; CMS1A2; CMS6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP28329
  • Protein Name
    Choline O-acetyltransferase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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SHSY5Y cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ChAT Polyclonal Antibody(bs-0042R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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Immunohistochemistry analysis in human placenta and liver tissues using AMAb91129 antibody. Corresponding CHAT RNA-seq data are presented for the same tissues.
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