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CHAT Antibody

CAB13244
Assay Genie
TargetCHAT
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    CHAT Rabbit pAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IHC,IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID1103
  • Target name
    CHAT
  • Target description
    choline O-acetyltransferase
  • Target synonyms
    acetyl CoA:choline O-acetyltransferase; CHOACTASE; choline acetylase; choline O-acetyltransferase; CMS1A; CMS1A2; CMS6
  • Host
    Rabbit
  • Protein IDP28329
  • Protein Name
    Choline O-acetyltransferase
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 2. IHC analysis of CHAT using anti-CHAT antibody (A01192-3). CHAT was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-CHAT Antibody (A01192-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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SHSY5Y cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ChAT Polyclonal Antibody(bs-0042R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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