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Anti-CHUK Antibody

144-02062
RayBiotech
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetCHUK
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-CHUK Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1147
  • Target name
    CHUK
  • Target description
    component of inhibitor of nuclear factor kappa B kinase complex
  • Target synonyms
    BPS2, IKBKA, IKK-1, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16, inhibitor of nuclear factor kappa-B kinase subunit alpha, I-kappa-B kinase 1, I-kappa-B kinase-alpha, IKK-a kinase, IkB kinase alpha subunit, Nuclear factor NFkappaB inhibitor kinase alpha, conserved helix-loop-helix ubiquitous kinase, transcription factor 16
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15111
  • Protein Name
    Inhibitor of nuclear factor kappa-B kinase subunit alpha
  • Scientific Description
    CHUK Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IKK alpha using anti-IKK alpha antibody (PB9110). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKK alpha antigen affinity purified polyclonal antibody (Catalog # PB9110) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IKK alpha at approximately 85 kDa. The expected band size for IKK alpha is at 85 kDa.
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