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Western Blot analysis of 293 cells using IKKalpha Polyclonal Antibody
Western Blot analysis of 293 cells using IKKalpha Polyclonal Antibody
Western Blot analysis of 293 cells using IKKalpha Polyclonal Antibody

CHUK Antibody

CSB-PA003008
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCHUK
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Overview

  • Supplier
    Cusabio
  • Product Name
    CHUK Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1147
  • Target name
    CHUK
  • Target description
    component of inhibitor of nuclear factor kappa B kinase complex
  • Target synonyms
    BPS2, IKBKA, IKK-1, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16, inhibitor of nuclear factor kappa-B kinase subunit alpha, I-kappa-B kinase 1, I-kappa-B kinase-alpha, IKK-a kinase, IkB kinase alpha subunit, Nuclear factor NFkappaB inhibitor kinase alpha, conserved helix-loop-helix ubiquitous kinase, transcription factor 16
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15111
  • Protein Name
    Inhibitor of nuclear factor kappa-B kinase subunit alpha
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of IKK alpha using anti-IKK alpha antibody (PB9110). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKK alpha antigen affinity purified polyclonal antibody (Catalog # PB9110) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IKK alpha at approximately 85 kDa. The expected band size for IKK alpha is at 85 kDa.
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