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IKK alpha Antibody (YA348, Rabbit)

HY-P80413
MedChem Express
TargetCHUK
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    IKK alpha Antibody (YA348, Rabbit)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID1147
  • Target name
    CHUK
  • Target description
    component of inhibitor of nuclear factor kappa B kinase complex
  • Target synonyms
    BPS2; conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; I-kappa-B kinase-alpha; IkB kinase alpha subunit; IKBKA; IKK1; IKKA; IKK-a kinase; IKK-alpha; inhibitor of nuclear factor kappa-B kinase subunit alpha; NFKBIKA; Nuclear factor NFkappaB inhibitor kinase alpha; TCF16; TCF-16; transcription factor 16
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    IKK alpha Antibody (YA348) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IKK alpha.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IKK alpha using anti-IKK alpha antibody (PB9110). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKK alpha antigen affinity purified polyclonal antibody (Catalog # PB9110) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IKK alpha at approximately 85 kDa. The expected band size for IKK alpha is at 85 kDa.
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