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IKK alpha antibody

GTX132964
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetCHUK
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Overview

  • Supplier
    GeneTex
  • Product Name
    IKK alpha antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.42 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1147
  • Target name
    CHUK
  • Target description
    component of inhibitor of nuclear factor kappa B kinase complex
  • Target synonyms
    BPS2; conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; I-kappa-B kinase-alpha; IkB kinase alpha subunit; IKBKA; IKK1; IKKA; IKK-a kinase; IKK-alpha; inhibitor of nuclear factor kappa-B kinase subunit alpha; NFKBIKA; Nuclear factor NFkappaB inhibitor kinase alpha; TCF16; TCF-16; transcription factor 16
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15111
  • Protein Name
    Inhibitor of nuclear factor kappa-B kinase subunit alpha
  • Scientific Description
    This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Hirsutanol A Attenuates Lipopolysaccharide-Mediated Matrix Metalloproteinase 9 Expression and Cytokines Production and Improves Endotoxemia-Induced Acute Sickness Behavior and Acute Lung Injury. Jan JS et al., 2019 Jun 17, Mar Drugs
    Read more

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Figure 1. Western blot analysis of IKK alpha using anti-IKK alpha antibody (PB9110). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKK alpha antigen affinity purified polyclonal antibody (Catalog # PB9110) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IKK alpha at approximately 85 kDa. The expected band size for IKK alpha is at 85 kDa.
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