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Anti-EME1 [7h2/1]

Ab01301-23.0
Absolute Antibody
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetEME1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-EME1 [7h2/1]
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: MTA31 7h2/1 antibody was used to detect EME1 in western blot analysis in the experiments assessing EME1 as a potential marker of cisplatin resistance in cancer therapy (Tomoda et al., 2009). WB: This antibody was used to detect EME1 on western blots after previous immunopurification procedure (Laguette et al., 2014). IHC: Other group conducted an immunohistochemistry experiment staining against EME1 in formalin-fixed paraffin embedded esophageal adenocarcinoma tissue samples using MTA31 7h2/1 antibody (Dwalt et al., 2014).
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    WB; IP; IF; IHC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    7h2/1
  • Gene ID146956
  • Target name
    EME1
  • Target description
    essential meiotic structure-specific endonuclease 1
  • Target synonyms
    crossover junction endonuclease EME1; essential meiotic endonuclease 1 homolog 1; essential meiotic endonuclease 1 homolog 2; hMMS4; homolog of yeast EME1 endonuclease; MMS4 homolog; MMS4L; SLX2 structure-specific endonuclease subunit homolog A; SLX2A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96AY2
  • Protein Name
    Crossover junction endonuclease EME1
  • Scientific Description
    This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    The antibody was raised against His-tagged recombinant Eme1 of human origin.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EME1 using anti-EME1 antibody (PB9804). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA Whole Cell Lysate, Lane 2: JURKAT Whole Cell Lysate, Lane 3: HUT Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EME1 antigen affinity purified polyclonal antibody (Catalog # PB9804) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EME1 at approximately 62KD. The expected band size for EME1 is at 62KD.
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