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ProductsBack/Anti-GAPDH Rabbit Monoclonal Antibody, HRP Conjugated
Western blot analysis of GAPDH expression in (1) Jurkat cell lysate; (2) A375 cell lysate; (3) Human hippocampus lysate; (4) Human fetal liver lysate; (5) COS-1 cell lysate; (6) Raw264.7 cell lysate; (7) Mouse kidney lysate; (8) PC-12 cell lysate; (9) Rat brain lysate
Western blot analysis of GAPDH expression in (1) Jurkat cell lysate; (2) A375 cell lysate; (3) Human hippocampus lysate; (4) Human fetal liver lysate; (5) COS-1 cell lysate; (6) Raw264.7 cell lysate; (7) Mouse kidney lysate; (8) PC-12 cell lysate; (9) Rat brain lysate
Western blot analysis of GAPDH expression in (1) Jurkat cell lysate; (2) A375 cell lysate; (3) Human hippocampus lysate; (4) Human fetal liver lysate; (5) COS-1 cell lysate; (6) Raw264.7 cell lysate; (7) Mouse kidney lysate; (8) PC-12 cell lysate; (9) Rat brain lysate

Anti-GAPDH Rabbit Monoclonal Antibody, HRP Conjugated

H00227
Boster Bio
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetGAPDH
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-GAPDH Rabbit Monoclonal Antibody, HRP Conjugated
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    A-7
  • Gene ID2597
  • Target name
    GAPDH
  • Target description
    glyceraldehyde-3-phosphate dehydrogenase
  • Target synonyms
    G3PD, GAPD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase, OCAS, p38 component, Oct1 coactivator in S phase, 38 Kd component, aging-associated gene 9 protein, epididymis secretory sperm binding protein Li 162eP, peptidyl-cysteine S-nitrosylase GAPDH
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04406
  • Protein Name
    Glyceraldehyde-3-phosphate dehydrogenase
  • Scientific Description
    Boster Bio Anti-GAPDH Rabbit Monoclonal Antibody, HRP Conjugated catalog # H00227. Tested in WB application. This antibody reacts with Human, Monkey, Mouse, Rat.
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Shang S, He Z, Hou W, et al. Molecular cloning, expression analysis and functional characterization of chicken cytochrome P450 27A1: A novel mitochondrial vitamin D(3) 25-hydroxylase. Poult Sci. 2023,102(8):102747. doi: 10.1016/j.psj.2023.102747
    Read this paper
  • Wei Y, Gao C, Cui J, et al. The response of electrochemical method to estrogen effect and the tolerance to culture factors: Comparison with MTT and cell counting methods. Anal Chim Acta. 2022,1233:340514. doi: 10.1016/j.aca.2022.340514
    Read this paper
  • Wei Y, Shen H, Gao C, et al. Electrochemical detection mechanism of estrogen effect induced by cadmium: The regulation of purine metabolism by the estrogen effect of cadmium. Chemosphere. 2023,311(Pt 2):136970. doi: 10.1016/j.chemosphere.2022.136970
    Read this paper
  • Chan L, Pang Y, Wang Y, et al. Genistein-induced mitochondrial dysfunction and FOXO3a/PUMA expression in non-small lung cancer cells. Pharm Biol. 2022,60(1):1876-1883. doi: 10.1080/13880209.2022.2123933
    Read this paper
  • Li Q, Jiang B, Zhang Z, et al. Serotonin system is partially involved in immunomodulation of Nile tilapia (Oreochromis niloticus) immune cells. Front Immunol. 2022,13:944388. doi: 10.3389/fimmu.2022.944388
    Read this paper
  • Jiao J, Zhao X, Wang Y, et al. Normal mesenchymal stem cells can improve the abnormal function of T cells in psoriasis via upregulating transforming growth factor-β receptor. J Dermatol. 2022,49(10):988-997. doi: 10.1111/1346-8138.16490
    Read this paper
  • Sun Q, Niu H, Wan C, et al. Memory-like response in platelet attenuates platelet hyperactivation in arterial thrombosis. Biochem Biophys Res Commun. 2022,612:154-161. doi: 10.1016/j.bbrc.2022.04.018
    Read this paper
  • Chu Q, Mao M, Bai Y, et al. Midazolam Attenuates Esketamine-Induced Overactive Behaviors in Mice Before the Sedation, but Not During the Recovery. Front Vet Sci. 2022,9:829747. doi: 10.3389/fvets.2022.829747
    Read this paper
  • Li Q, Jiang B, Zhang Z, et al. SP protects Nile tilapia (Oreochromis niloticus) against acute Streptococcus agalatiae infection. Fish Shellfish Immunol. 2022,123:218-228. doi: 10.1016/j.fsi.2022.03.002
    Read this paper
  • Fu XF, Zhao HC, Yang CL, et al. MicroRNA-203-3p inhibits the proliferation, invasion and migration of pancreatic cancer cells by downregulating fibroblast growth factor 2. Oncol Lett. 2021,22(2):626. doi: 10.3892/ol.2021.12887
    Read this paper

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Figure 1. Western blot analysis of GAPDH using anti-GAPDH antibody (A00227-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human CCRF-CEM whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAPDH antigen affinity purified polyclonal antibody (Catalog # A00227-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.
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